The turbulence characteristics in flow over and within the interface of two-dimensional dunes are investigated experimentally. Besides the spatial flow and turbulence quantities, their double-averaged profiles are also analyzed. The flow over dunes is recognized to be a wake-interference flow, where the decelerated flow at the immediate downstream of the crest causes the kolk-boil effect. The flow reattachment can be explained from the perspective of the CoandÄ effect. The inner boundary layer edge follows the locus of the inflection points of velocity profiles having a velocity defect. The Reynolds shear stress profiles attain their respective peaks along this locus. In addition, the dispersive shear stress initiates from the edge of the form-induced sublayer being negative, indicating a spatially decelerated flow. The third-order correlations reveal that an inrush of rapidly moving fluid streaks coupled with a downward-downstream Reynolds stress diffusion prevails within the interfacial sublayer, while an arrival of slowly moving fluid streaks coupled with an upward-upstream stress diffusion governs the flow zone above the crest. The turbulent kinetic energy (TKE) flux results corroborate the similar findings. Concerning the TKE budget, the dispersive kinetic energy diffusion is found to be substantial within the roughness sublayer. The budget terms exhibit their respective peaks near the crest. The production rate is greater than the dissipation rate. However, the TKE diffusion and pressure energy diffusion rates are negative in the interfacial sublayer. The bursting analysis endorses that the sweeps and ejections govern within the interfacial sublayer and the flow zone above the crest, respectively. © 2020 Author(s).