Purpose: Biofilm growth changes the sediment properties and the characteristics of the bed, which further influences the interactions between the flow and the sediment bed, such as the turbulence characteristics of the flow and the erosion response of the sediment to hydrodynamic forces. In this study, the effects of biofilm on the turbulence characteristics of the flow and bio-sediment suspension are investigated. Materials and methods: Cohesive sediments were collected from the bottom of the Three Gorges Reservoir, which have a median size of less than 0.1 mm. Flume experiments were conducted for the erosion of the sediment bed with and without biofilm under the same flow rate. Acoustic Doppler Velocimetry (ADV) was used to measure the velocities along the depth, based on which the distribution of Reynolds shear stress, time-average velocity, turbulence intensities, turbulence kinetic energy flux and budgets, and bursting events of the flow were determined. Meanwhile, the biofilm effects on the physical characteristics of sediments, such as the diameter, density, and falling velocity, were summarized from previous studies. Then, the changes of the vertical distribution of suspended sediment concentration and the near-bed concentration were evaluated. Results and discussion: After biofilm growth, the time-averaged velocity increases by approximately 6.7% under the low flow rate condition with a flat bed, and by more than 20% under the high flow rate condition with a deformed bed. The vertical distribution of the turbulence intensity becomes more uniform under the high flow rate condition. However, the changes of the turbulence kinetic energy flux and budgets due to biofilm are hardly observed by the scattered measurement data, and more experiments need to be conducted in the future. Conclusions: Biofilm exerts an influence on the turbulence characteristics, mostly by affecting the deformation extent of the sediment bed. Meanwhile, the changes of the physical properties of sediments due to biofilm significantly influence the transport of suspended sediment. © 2017, Springer-Verlag GmbH Germany.