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Single-molecule fluorescence resonance energy transfer in molecular biology
, L.E. Pulido, S. Kasal, J. Huang
Published in Royal Society of Chemistry
PMID: 27883140
Volume: 8
Issue: 48
Pages: 19928 - 19944
Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying the conformation dynamics and interactions of individual biomolecules. In this review, we describe the concept and principle of smFRET, illustrate general instrumentation and microscopy settings for experiments, and discuss the methods and algorithms for data analysis. Subsequently, we review applications of smFRET in protein conformational changes, ion channel open-close properties, receptor-ligand interactions, nucleic acid structure regulation, vesicle fusion, and force induced conformational dynamics. Finally, we discuss the main limitations of smFRET in molecular biology. © The Royal Society of Chemistry.
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JournalData powered by TypesetNanoscale
PublisherData powered by TypesetRoyal Society of Chemistry