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Sensitivity enhancement of electrochemical biosensor for point of care (POC) applications: Vi antigen detection as a case study
R.K. Maurya, M.A. Alam, N. Ahamad, K. Kishore, R. Prajesh, M. Choudhary, V. Bhalla,
Published in IOP Publishing Ltd
Volume: 168
Issue: 1
A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide ([Fe(CN)6]-3/-4) a redox couple, at room temperature (~25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ~332% (from 3.125 nA μM-1 to 13.5 nA μM-1) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ~15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper. © 2021 The Electrochemical Society ("ECS").
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JournalData powered by TypesetJournal of the Electrochemical Society
PublisherData powered by TypesetIOP Publishing Ltd