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Repeated random mutagenesis of α-amylase from Bacillus licheniformis for improved pH performance
R. Priyadharshini, , D. Hemalatha, P. Gunasekaran
Published in
PMID: 21193826
Volume: 20
Issue: 12
Pages: 1696 - 1701
The α-amylases activity was improved by random mutagenesis and screening. A region comprising residues from the position 34-281 was randomly mutated in B. licheniformis α-amylase (AmyL), and the library with mutations ranging from low, medium, and high frequencies was generated. The library was screened using an effective liquid-phase screening method to isolate mutants with an altered pH profile. The sequencing of improved variants indicated 2-5 amino acid changes. Among them, mutant TP8H5 showed an altered pH profile as compared with that of wild type. The sequencing of variant TP8H5 indicated 2 amino acid changes, Ile157Ser and Trp193Arg, which were located in the solvent accessible flexible loop region in domain B.
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JournalJournal of Microbiology and Biotechnology