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Quantitative digital image analysis of chromogenic assays for high throughput screening of α-amylase mutant libraries
, R. Priyadharshini, P. Gunasekaran
Published in
PMID: 19360387
Volume: 31
Issue: 8
Pages: 1197 - 1201
An image analysis-based method for high throughput screening of an α-amylase mutant library using chromogenic assays was developed. Assays were performed in microplates and high resolution images of the assay plates were read using the Virtual Microplate Reader (VMR) script to quantify the concentration of the chromogen. This method is fast and sensitive in quantifying 0.025-0.3 mg starch/ml as well as 0.05-0.75 mg glucose/ml. It was also an effective screening method for improved α-amylase activity with a coefficient of variance of 18%. © Springer Science+Business Media B.V. 2009.
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JournalBiotechnology Letters