This article describes the psychometric properties of a semantic differential scale developed to evaluate the attitudes towards menstruation among Roman Catholics in India, which is referred to as Menstrual Semantic Differential Scale (MSDS). For this purpose, the south Indian state of Kerala was chosen as the location. First, exploratory factor analysis was conducted on the data collected from a sample of participants of different ages, gender, and caste groups. The analysis produced a 10 item scale with a tri-factorial structure that explained 74 percent of the variance. Subsequently, confirmatory factor analysis on a different sample established that the three-factor model was a good fit. Moreover, Cronbach’s alpha coefficients of the subscales ranged from 0.73 to 0.93, confirming the acceptable reliability of the instrument. The findings suggest that the MSDS is a reliable and valid measure for assessing attitudes towards menstruation among Roman Catholics in India. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.