Walking the line: Selective photodestruction of nitrilotriacetic acid moieties on a poly(ethylene glycol) polymer brush by a light-induced Fenton reaction enabled the functional organization of motor proteins into micropatterns (see schematic illustration). Microtubules were selectively captured on the structures, and adenosine triphosphate dependent transport along lines was observed. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.