Header menu link for other important links
X
Mechanical characterization of erythrocyte-derived optical microparticles by quantitative phase imaging and optical tweezers
T. Lu, J.T. Mac, R. Tweini, K. Tran, , B. Anvari
Published in SPIE
2019
Volume: 10887
   
Abstract
We have fabricated constructs from erythrocytes that contain the near-infrared (NIR) dye, indocyanine green (ICG). We refer to these constructs as NIR erythrocyte mimicking transducers (NETs). Mechanical properties of NETs can play an important role in the circulation kinetics and biodistribution of these particles. We characterize the mechanical properties of erythrocytes, hemoglobin-depleted erythrocytes ghosts (EGs), and micron-sized NETs (μNETs) through analysis of membrane fluctuations measured by quantitative phase imaging, and forces associated with membrane tethers pulled by optical tweezers. EGs were prepared from erythrocytes by hypotonic treatment. μNETs were prepared through hypotonic loading of 25 μM ICG into EGs. Quantitative phase images were obtained by a common-path interferometric phaseshifting system. Approximating the membrane as a sheet of springs, we estimated the stiffness of the membrane of erythrocytes, EGs, and μNETs as 3.0 ± 0.6 pN/μm, 6.5 ± 2.1 pN/μm, and 8.0 ± 2.1 pN/μm. Optical tweezers experiments yielded a similar trend. Differences in membrane stiffness suggest that the circulation dynamics of μNETs may be altered as compared to native erythrocytes. © 2019 SPIE.
About the journal
JournalData powered by TypesetProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherData powered by TypesetSPIE
ISSN16057422