Background: Hydroxyapatite (HAP) has been a principal mineral phase in bone cell and tooth because of several favourable properties like anti-carcinogenicity, good mechanical strength, biocompatibility etc. ZnO being an inorganic material possesses unique properties such as antisepticity, low thermal expansion coefficient, low thermal conductivity and antimicrobial properties, all of which find prominent applications in dental materials. In this regard, we have prepared a novel biomaterial blending ZnO and HAP into a nano-composite which can be incorporated directly into the restorative glass ionomer cement (GIC) to enhance its mechanical and antibacterial properties. Results: We have fabricated the ZnO-HAP nano composite using a wet chemical route through ultrasonication process. Characterization of this nano composite has been carried out with Dynamic Light Scattering (DLS), Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infra Red Spectroscopy (FTIR) and Micro-hardness testing etc. Further, the antimicrobial properties of ZnO-HAP nano composites embedded in GIC has been investigated for the E. Coli DH5α and Streptococcus mutans and we have found out the composite to significantly retard microbial activity. Copyright © 2015 American Scientific Publishers.