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Integrated sorting, concentration and real time PCR based detection system for sensitive detection of microorganisms
M. Nayak, D. Singh, H. Singh, R. Kant, , S.S. Pandey, S. Mandal, G. Ramanathan, S. Bhattacharya
Published in
PMID: 24253282
Volume: 3
The extremely low limit of detection (LOD) posed by global food and water safety standards necessitates the need to perform a rapid process of integrated detection with high specificity, sensitivity and repeatability. The work reported in this article shows a microchip platform which carries out an ensemble of protocols which are otherwise carried in a molecular biology laboratory to achieve the global safety standards. The various steps in the microchip include pre-concentration of specific microorganisms from samples and a highly specific real time molecular identification utilizing a q-PCR process. The microchip process utilizes a high sensitivity antibody based recognition and an electric field mediated capture enabling an overall low LOD. The whole process of counting, sorting and molecular identification is performed in less than 4 hours for highly dilute samples.
About the journal
JournalScientific Reports