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Genome sequencing of a mung bean plant growth promoting strain of P. aeruginosa with biocontrol ability
D. Illakkiam, , P. Ponraj, J. Rajendhran, P. Gunasekaran
Published in Hindawi Limited
Volume: 2014
Pseudomonas aeruginosa PGPR2 is a mung bean rhizosphere strain that produces secondary metabolites and hydrolytic enzymes contributing to excellent antifungal activity against Macrophomina phaseolina, one of the prevalent fungal pathogens of mung bean. Genome sequencing was performed using the Ion Torrent Personal Genome Machine generating 1,354,732 reads (6,772,433 sequenced bases) achieving ∼25-fold coverage of the genome. Reference genome assembly using MIRA 3.4.0 yielded 198 contigs.The draft genome of PGPR2 encoded 6803 open reading frames, of which 5314 were genes with predicted functions, 1489 weregenes of known functions, and 80 were RNA-coding genes. Strain specific and core genes of P. aeruginosa PGPR2 that are relevantto rhizospheric habitat were identified by pangenome analysis. Genes involved in plant growth promoting function such as synthesisof ACC deaminase, indole-3-acetic acid, trehalose, mineral scavenging siderophores, hydrogen cyanide, chitinases, acyl homoserinelactones, acetoin, 2,3-butanediol, and phytases were identified. In addition, niche-specific genes such as phosphate solubilising 3-phytase, adhesins, pathway-specific transcriptional regulators, a diguanylate cyclase involved in cellulose synthesis, a receptor forferrienterochelin, a DEAD/DEAH-box helicase involved in stress tolerance, chemotaxis/motility determinants, an HtpX protease,and enzymes involved in the production of a chromanone derivative with potent antifungal activity were identified. Copyright © 2014 Devaraj Illakkiam et al.
About the journal
JournalInternational Journal of Genomics
PublisherHindawi Limited