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Fabrication of biotin functionalised SiO2 EM grid for studying biotin tagged biomolecules
I. Chakraborty, A. Saha,
Published in
Volume: 52
Issue: 8-9
Pages: 1026 - 1030
A biotin functionalised SiO2 electron microscope grid has been developed for studying various biotin/avidin tagged biomolecules onto the grid surface under electron microscope. As an application, we have shown successful immobilisation of streptavidin gold nanoparticles and biotinylated microtubules through biotin-streptavidin-biotin interaction. This grid will be useful for studying the structures of various biotin-tagged proteins and their complexes under electron microscope.
About the journal
JournalIndian Journal of Chemistry - Section A Inorganic, Physical, Theoretical and Analytical Chemistry