Several successful adversarial attacks have demonstrated the vulnerabilities of deep learning algorithms. These attacks are detrimental in building deep learning based dependable AI applications. Therefore, it is imperative to build a defense mechanism to protect the integrity of deep learning models. In this paper, we present a novel "defense layer" in a network which aims to block the generation of adversarial noise and prevents an adversarial attack in black-box and gray-box settings. The parameter-free defense layer, when applied to any convolutional network, helps in achieving protection against attacks such as FGSM, L2, Elastic-Net, and DeepFool. Experiments are performed with different CNN architectures, including VGG, ResNet, and DenseNet, on three databases, namely, MNIST, CIFAR-10, and PaSC. The results showcase the efficacy of the proposed defense layer without adding any computational overhead. For example, on the CIFAR-10 database, while the attack can reduce the accuracy of the ResNet-50 model to as low as 6.3%, the proposed "defense layer" retains the original accuracy of 81.32%. © 2020 IEEE.