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Detergent-induced aggregation of an amyloidogenic intrinsically disordered protein
S. Arya, P. Dogra, , S. Mukhopadhyay
Published in Springer India
Volume: 129
Issue: 12
Pages: 1817 - 1827
Abstract : Intrinsically disordered proteins (IDPs) belong to an important class of proteins that do not fold up spontaneously. The conformational flexibility of IDPs allows them to adopt a wide range of conformations depending upon their biochemical environment. Many IDPs undergo profound conformational conversion that is often coupled to amyloid aggregation in the presence of negatively charged lipid membranes. Here, we show the effect of a well-known anionic lipid mimetic, sodium dodecyl sulfate (SDS), on the aggregation mechanism of a model amyloidogenic IDP, namely, bovine κ -casein. In the absence of SDS, the aggregation kinetics of reduced and carboxymethylated (RCM) κ -casein followed a nucleation dependent polymerization model that comprises both lag- and assembly phases. On the contrary, in the presence of sub-micellar concentration of SDS, the aggregation kinetics did not exhibit a lag phase and appears to follow a non-nucleation pathway. Additionally, the morphologies of the aggregates formed in the absence and presence of SDS were found to be different. In the absence of SDS, κ -casein aggregation proceeded to typical amyloid fibrils, whereas, in the presence of SDS, the aggregation yielded large oligomers. Our results provide important molecular insights into the aggregation mechanism that can be utilized for the designing of novel protein/amyloid based nanomaterials with desired properties. Graphical Abstract : Synopsis: An amyloidogenic intrinsically disordered protein, κ -casein aggregates into amyloid fibrils via a typical nucleation-dependent kinetics that exhibits a lag-phase. However, in the presence of an anionic surfactant, such as sodium dodecyl sulfate, κ -casein aggregation is devoid of a lag-phase and yields large oligomers that do not convert into amyloid fibrils. © 2017, Indian Academy of Sciences.
About the journal
JournalData powered by TypesetJournal of Chemical Sciences
PublisherData powered by TypesetSpringer India