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Cellphone camera imaging of a periodically patterned chip as a potential method for point-of-care diagnostics
, R.G. Reifenberger, G.U. Kulkarni
Published in American Chemical Society
PMID: 24564576
Volume: 6
Issue: 6
Pages: 3923 - 3929
In this study, we demonstrate that a disposable chip periodically patterned with suitable ligands, an ordinary cellphone camera, and a simple pattern recognition software, can potentially be used for quantitative diagnostics. A key factor in this demonstration is the design of a calibration grid around the chip that, through a contrast transfer process, enables reliable analysis of the images collected under variable ambient lighting conditions. After exposure to a dispersion of amine terminated silica beads used as analyte mimicking pathogens, an epoxy-terminated glass substrate microcontact printed with octadecyltrichlorosilane (250 μm periodicity) developed a characteristic pattern of beads which could be easily imaged with a cellphone camera of 3.2 MP pixels. A simple pattern recognition algorithm using fast Fourier transform produced a quantitative estimate of the analyte concentration present in the test solution. In this method importantly, neither the chip fabrication process nor the fill-factor of the periodic pattern need be perfect to arrive at a conclusive diagnosis. The method suggests a viable platform that may potentially find use in fault-tolerant and robust point-of-care diagnostic applications. © 2014 American Chemical Society.
About the journal
JournalData powered by TypesetACS Applied Materials and Interfaces
PublisherData powered by TypesetAmerican Chemical Society