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A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites
, C.-S. Chiang, J.-I. Chao, C.-J. Yuan, S.-Y. Lin, K.C. Hwang
Published in Elsevier Ltd
PMID: 24973297
Volume: 35
Issue: 28
Pages: 8261 - 8272
Gene therapy provides a new hope for previously "incurable" diseases. Low gene transfection efficiency, however, is the bottle-neck to the success of gene therapy. It is very challenging to develop non-viral nanocarriers to achieve ultra-high gene transfection efficiencies. Herein, we report a novel design of "tight binding-but-detachable" lipid-nanoparticle composite to achieve ultrahigh gene transfection efficiencies of 60~82%, approaching the best value (~90%) obtained using viral vectors. We show that Fe@CNPs nanoparticles coated with LP-2000 lipid molecules can be used as gene carriers to achieve ultra-high (60-80%) gene transfection efficiencies in HeLa, U-87MG, and TRAMP-C1 cells. In contrast, Fe@CNPs having surface-covalently bound N,N,N-trimethyl-N-2-methacryloxyethyl ammonium chloride (TMAEA) oligomers can only achieve low (23-28%) gene transfection efficiencies. Similarly ultrahigh gene transfection/expression was also observed in zebrafish model using lipid-coated Fe@CNPs as gene carriers. Evidences for tight binding and detachability of DNA from lipid-nanoparticle nanocarriers will be presented. © 2014 Elsevier Ltd.
About the journal
JournalData powered by TypesetBiomaterials
PublisherData powered by TypesetElsevier Ltd