In this study, Pauli-Blocking has been observed in a 0D/2D MoS2 quantum dots/graphene (MoS2-QDs/graphene) hybrid system. For the observation of room temperature Pauli-Blocking in the 0D/2D system, a photodetector device based on n-type MoS2-QDs and CVD grown graphene has been fabricated using a facile and lithography free technique. The current-voltage characteristics of the device have been performed at room temperature. The fabricated device shows a negative response under visible light (λ ∼ 400 to 700 nm) illumination. The dark to photo current ratio of the device shows variation up to two orders of magnitude. This negative response, which results decrease in current under visible light illumination, may be attributed to the Pauli-Blocking due to high absorbance of photon energy in visible light range. Furthermore, it is believed that the present study may provide an insight into understanding the Pauli-Blocking in 0D/2D hybrid system at room temperature. © 2018 Author(s).